猪Fosl2基因克隆、结构预测及其mRNA发育性表达变化

doi:10.11843/j.issn.0366-6964.2015.02.006

Abstract

Abstract:

The study was conducted to clone and analyze CDS sequence of pig Fosl2 gene，and to investigate the developmental expression of Fosl2 in pig various tissues(heart，liver，spleen，lung，kidney，the longissimus muscle and backfat) at different stages.In this study，Yunan Black pig was used as experimental animal to clone the CDS region of Fosl2 gene by RT-PCR，the protein structure were predicted by bioinformatics，meanwhile，qRT-PCR was used to analyze developmental expression of Fosl2 gene in pig from 7- to 180-day-old.The results showed that the CDS of pig Fosl2 gene was 984 bp，encoding 327 amino acids.It had no signal peptide in peptide chain，but had a transmembrane domain and a basic-domain leucine-zipper，which were from 17 to 33 amino acids and from 122 to 187 amino acids,respectively.The protein of pig Fosl2 had high conservatism among mammal，and it was transmembrane and non-secretory protein.The Fosl2 was widely expressed in tissues，the expression of Fosl2 in lung was higher than other tissues at the same stage，it was deduced that pig Fosl2 might be related to the process of lung development；the variation trend in backfat showed that Fosl2 might had the function enhancing adipogenesis and then regulate lean meat percentage.Therefore，the results of this study may lay foundation for further research on the structure and the role of Fosl2 gene in physiogenesis in pig.

CLC Number:

S828.2

FAN Xing-xing，LI Xin-jian，LI Gai-ying，GUO Ji-li，HAN Xue-lei，Lü Gang，REN Guang-zhi. Gene Cloning，Bioinformatics Analysis and Developmental Expression of Fosl2 Gene in Pig[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(2): 211-218.

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Gene Cloning，Bioinformatics Analysis and Developmental Expression of Fosl2 Gene in Pig

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